Human microdissected liver tissue sample (GEO)
7137_IZ_RRBS
hepatocyte
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM2819645
https://seek.lisym.org/sops/22
intermediate
18 October 2017
genomic DNA
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Created: 13th Jul 2021 at 13:50
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Liver Systems Medicine : striving to develop non-invasive methods for diagnosing and treating NAFLD by combining mathematical modeling and biological research. LiSyM, is a multidisciplinary research network, in which molecular and cell biologists, clinical researchers, pharmacologists and experts in mathematical modeling examine the liver in its entirety. LiSyM research focuses on the metabolic liver disease non-alcoholic fatty liver disease (NAFLD), which includes non-alcoholic steatohepatitis ...
Projects: LiSyM Core Infrastructure and Management (LiSyM-PD), LiSyM Pillar I: Early Metabolic Injury (LiSyM-EMI), LiSyM Pillar II: Chronic Liver Disease Progression (LiSyM-DP), LiSyM Pillar III: Regeneration and Repair in Acute-on-Chronic Liver Failure (LiSyM-ACLF), LiSyM Pillar IV: Liver Function Diagnostics (LiSyM-LiFuDi), Model Guided Pharmacotherapy In Chronic Liver Disease (LiSyM-MGP), Molecular Steatosis - Imaging & Modeling (LiSyM-MSIM), The Hedgehog Signalling Pathway (LiSyM-JGMMS), Multi-Scale Models for Personalized Liver Function Tests (LiSyM-MM-PLF), LiSyM PALs, Project Management PTJ, LiSyM network, LiSyM Scientific Leadership Team (LiSyM-LT)
Web page: https://www.lisym.org/
One of the tasks of the healthy liver is to store fat. Yet, at some stage, too much fat makes the liver sick. One critical time point occurs when a healthy fatty liver becomes inflamed and progresses to steatohepatitis, or NASH. LiSyM-Pillar I will identify what events lead to this transition. Does it occur in all parts of the liver? Which molecules indicate that it is taking place? Can the degeneration be stopped or undone - and if so, how?
Programme: LiSyM: Liver Systems Medicine
Public web page: http://www.lisym.org/our-work/pillar-research/zones-of-the-liver
Start date: 1st Jan 2016
Organisms: Mus musculus, Rattus rattus, Rattus norvegicus, Homo sapiens
To investigate the underlying molecular principles of metabolic and morphogenic zonation of the human liver lobule, we generated an integrated epigenetic map across three zones (pericentral, intermediate and periportal) by methylation and transcriptomic analysis of hepatocytes captured by laser micro-dissection. We observe a deep link between epigenetic zonation of human liver and a zonated expression of metabolic and morphogenic pathways: Key transcriptionally zonated enzymes in xenobiotic and ...
Submitter: Mario Brosch
Studies: Integrated epigenetic map across three hepatic zones (pericentral, inte...
Assays: DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., Transcriptomic analysis of hepatocytes ( hepatic zone: intermediate (IZ), Transcriptomic analysis of hepatocytes ( hepatic zone: periportal (PP), Transcriptomic analysis of hepatocytes ; hepatic zone: pericentral (CV)
Snapshots: No snapshots
The study comprises 19 human liver biopsy donors divided into the groups normal control (NC = 7012, 7173, 7194, 7279), healthy obese (HO = 6758, 6922, 7213, 7230, 7252), bland steatosis (STEA = 6967, 7137, 7172, 7181, 7251) and early NASH (EARLY = 6610, 7041, 7157, 7188, 7344).
Hepatocytes captured by laser microdissection were obtained from three hepatic zones (pericentral, intermediate and periportal) and subjected to reduced representation bisulfite sequencing and RNA-seq resulting in 114 ...
Submitter: Mario Brosch
Investigation: Epigenomic analysis of micro-dissected human liver
Assays: DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., Transcriptomic analysis of hepatocytes ( hepatic zone: intermediate (IZ), Transcriptomic analysis of hepatocytes ( hepatic zone: periportal (PP), Transcriptomic analysis of hepatocytes ; hepatic zone: pericentral (CV)
Snapshots: No snapshots
DNA-methylation at binding sites of uniformly expressed transcription factors in human hepatocytes ; hepatic zone: intermediate (IZ)
Submitter: Olga Krebs
Assay type: Methylation Profiling
Technology type: Technology Type
Investigation: Epigenomic analysis of micro-dissected human liver
Organisms: Homo sapiens
SOPs: No SOPs
Data files: Human DNA methylation data set GSM2819639 ..., Human DNA methylation data set GSM2819641 ..., Human DNA methylation data set GSM2819642 ..., Human DNA methylation data set GSM2819643 ..., Human DNA methylation data set GSM2819647 ..., Human DNA methylation data set GSM2819640 s..., Human DNA methylation data set GSM2819644 s..., Human DNA methylation data set GSM2819645 s..., Human DNA methylation data set GSM2819646 s..., Human DNA methylation data set GSM2819648 s..., Human DNA methylation data set GSM2819650 s..., Human DNA methylation data set GSM2819651 s..., Human DNA methylation data set GSM2819652 s..., Human DNA methylation data set GSM2819653 s..., Human DNA methylation data set GSM2819654 s..., Human DNA methylation data set GSM2819655 s..., Human DNA methylation data set GSM2819656 s..., Human DNA methylation data set GSM2819657 s..., HumanDNA methylation data set GSM2819649 st...
Snapshots: No snapshots
sample data
Creator: Mario Brosch
Submitter: Mario Brosch
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
Taxonomy URI: http://purl.bioontology.org/ontology/NCBITAXON/9606
Synonyms: man
Human microdissected liver tissue sample
Published sample ID (String) *, Lab sample ID (String) *, Tissue (Controlled Vocabulary - Liver cell types) *, Organism (NCBI ID) , Sample web link (Web link) , Link to SOP used (Web link) , hepatic zone (Controlled Vocabulary - Hepatic zones in human hepatocytes) , Sample creation date (Date) , Molecule type (String)