The Hedgehog Signalling Pathway (LiSyM-JGMMS)

Liver Systems Medicine : striving to develop non-invasive methods for diagnosing and treating NAFLD by combining mathematical modeling and biological research. LiSyM, is a multidisciplinary research network, in which molecular and cell biologists, clinical researchers, pharmacologists and experts in mathematical modeling examine the liver in its entirety. LiSyM research focuses on the metabolic liver disease non-alcoholic fatty liver disease (NAFLD), which includes non-alcoholic steatohepatitis ...

Liver macrophages (LMs) play a central role in acute and chronic liver pathologies. In our study, we sought to gain further insight into the role of LMs in different liver pathologies and into their characteristics after isolation from liver tissue. Here we will characterize LMs in human liver tissue sections using immunohistochemistry and bioinformatic image analysis. We investigate liver tissues characterized by antiinflammatory LMs which show a homogeneous distribution and increased cell numbers ...

In this study, we investigated human LMs in tissue sections as well as isolated from human liver tissues which were characterized both in suspension and in adherent cultures to find out whether the inflammatory state of primary human LMs corresponds to patients and disease characteristics and whather it is possible to maintain the macrophage pattern in suspension as well as in adherent cultures with just a few limitations. (CCA: cholangiocarcinoma, CRLM: colorectal liver metastasis, FNH: focal ...

LMs were characterized in human liver tissue sections using immunohistochemistry and bioinformatic image analysis. Isolated cells were characterized in suspension using FACS analyses and in culture using

Metabolic capacities of the Intermediate zone

LFQ intensities of the model protein subset with imputed missing values highlighted in yellow.

List of proteins that are significantly higher abundant in the portal zone (sheet 1: from the subset of model proteins, sheet 3: from whole proteome) or in the central zone (sheet 2: from the subset of model proteins, sheet 4: from whole proteome).

LFQ intensities and protein IDs of the 3736 proteins of the proteome for the 18 samples.

Scaling of the maximal enzyme activities for the 226 modelled processes for portal and central zone of the 6 mice.

Raw data metabolom study Kolbe et al.

Raw data metabolomics Kolbe et al.

The spatial separation of metabolic functions along the porto-central axis within the liver lobule is termed liver zonation. The zonated metabolic endowment of hepatocytes along the sinusoid are partially influenced by adaption to changing contents of nutrients and oxygen, but is mainly regulated by the morphogenic Wnt/β-Catenin signaling pathway. How morphogens contribute to the lobular distribution of metabolic pathways is part of an evolving field in science since the last decade. For long, ...

Poster

Poster

Poster

Poster for 1st annual LiSyM Meeting

Morphogenic impact on liver zonation and metabolism

Power Point description

The meeting will provide a great opportunity for discussion between modellers, experimentalists and clinicians and to organize and harmonize our future work within the LiSyM network on the grassroot level.

This summit will cover both the basic and translational / clinical aspects of NAFLD, with a specific focus on novel drug target and prognostic signature development.

Key sessions include all crucial pathophysiological aspects currently known to affect development and progression of NAFLD, such as "lipid metabolism and lipotoxicity" and "insulin resistance, insulin and hormonal signaling". Furthermore, oxidative stress, ER stress, and mitochondrial dysfunction will be addressed. On the second day, ...

ICSB continues to be the flagship conference of the International Society for Systems Biology (ISSB). The conference will cover a variety of themes from Fundamental Biological Sciences, Network Analysis, to Metabolic Engineering and Cancer Biology. The conference will also include a variety of engaging plenary lectures, parallel sessions, poster sessions, and interactive networking opportunities.

Abstract submission deadline: May 13th, 2017

The International Conference on Systems Biology of Human Disease (SBHD) is a transatlantic event and communication platform for scientific exchange that takes place alternately between the United States and Germany.

SBHD 2017 is continuing a conference series launched by Peter Sorger and colleagues from Harvard Medical School in 2008. The conference focus is on mammalian systems biology, particularly on its application to human disease and therapy.

This event is a jointly organized Data Management PALs Meeting that involves PALs from the German LiSyM (Liver Systems Medicine) network and from the ERASysAPP (ERA-Net for Systems Biology Applications) initiative. PALs (Project Area Liaisons) are the front line experts of the data management teams of these projects. They act as data management advocates and help gathering user requirements and testing potential data management solutions. An important part of the meeting will be to gather the ...