Epigenomic analysis of micro-dissected human liver

Liver Systems Medicine : striving to develop non-invasive methods for diagnosing and treating NAFLD by combining mathematical modeling and biological research. LiSyM, is a multidisciplinary research network, in which molecular and cell biologists, clinical researchers, pharmacologists and experts in mathematical modeling examine the liver in its entirety. LiSyM research focuses on the metabolic liver disease non-alcoholic fatty liver disease (NAFLD), which includes non-alcoholic steatohepatitis ...

One of the tasks of the healthy liver is to store fat. Yet, at some stage, too much fat makes the liver sick. One critical time point occurs when a healthy fatty liver becomes inflamed and progresses to steatohepatitis, or NASH. LiSyM-Pillar I will identify what events lead to this transition. Does it occur in all parts of the liver? Which molecules indicate that it is taking place? Can the degeneration be stopped or undone - and if so, how?

The study comprises 19 human liver biopsy donors divided into the groups normal control (NC = 7012, 7173, 7194, 7279), healthy obese (HO = 6758, 6922, 7213, 7230, 7252), bland steatosis (STEA = 6967, 7137, 7172, 7181, 7251) and early NASH (EARLY = 6610, 7041, 7157, 7188, 7344).

Hepatocytes captured by laser microdissection were obtained from three hepatic zones (pericentral, intermediate and periportal) and subjected to reduced representation bisulfite sequencing and RNA-seq resulting in 114 ...

Transcriptomic analysis of hepatocytes captured by laser micro-dissection from hepatic zone: pericentral (CV)

DNA-methylation at binding sites of uniformly expressed transcription factors in human hepatocytes ; hepatic zone: pericentral (CV)

Transcriptomic analysis of hepatocytes captured by laser micro-dissection from hepatic zone: intermediate (IZ)

Transcriptomic analysis of hepatocytes captured by laser micro-dissection from hepatic zone: periportal (PP)

DNA-methylation at binding sites of uniformly expressed transcription factors in human hepatocytes ; hepatic zone: intermediate (IZ)

DNA-methylation at binding sites of uniformly expressed transcription factors in human hepatocytes ; periportal (PP)

Human RNA-Seq data set GSM2819712 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819698 stored in NCBI (GEO)

liver tissue sample : 6922_IZ_RNA

Human RNA-Seq data set GSM2819714 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819713 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819711 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819710 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819709 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819695 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819694 stored in NCBI (GEO)

Human RNA-Seq data set GSM2819693 stored in NCBI (GEO)

SOP : liver tissue microdissection for RNA sequencing