Investigations

Due to increasing incidence and high mortality rates, hepatocellular carcinoma (HCC) is an important health problem worldwide. However, its etiopathology and development are poorly understood. While liver metabolism is organized in spatially specific zones, little is known how the zonation is associated with the onset and propagation of liver diseases. To this end, we applied laser capture microdissection (LCM) technique to excise histologically specific features of liver tissues, healthy and ...

We apply multiphoton imaging, 3D digital reconstructions and computational simulations to generate spatially-resolved geometrical and functional models of human liver tissue at different stages of non-alcoholic fatty liver disease (NAFLD).

Macrophages play an important role in maintaining liver homeostasis and regeneration. Here we investigate how different macrophage populations of the liver differ in terms of their activation state and which other liver cell populations may play a role in regulating the same.

LiSyM-Cancer project data for integration into public repositories based on

FAIR principles

Upon stimulation of cells with transforming growth factorb(TGF-b),Smad proteins form trimeric complexes and activate a broad spectrum of target genes. It remains unresolved which of the possible Smad complexes are formed in cellular contexts and how these contribute to gene expression. Combining quantitative mass spectrometry with a computational selection strategy, we investigate Smad complexes in the mouse hepatoma cell line Hepa1-6.

To investigate the underlying molecular principles of metabolic and morphogenic zonation of the human liver lobule, we generated an integrated epigenetic map across three zones (pericentral, intermediate and periportal) by methylation and transcriptomic analysis of hepatocytes captured by laser micro-dissection. We observe a deep link between epigenetic zonation of human liver and a zonated expression of metabolic and morphogenic pathways: Key transcriptionally zonated enzymes in xenobiotic and ...

Liver macrophages (LMs) play a central role in acute and chronic liver pathologies. In our study, we sought to gain further insight into the role of LMs in different liver pathologies and into their characteristics after isolation from liver tissue. Here we will characterize LMs in human liver tissue sections using immunohistochemistry and bioinformatic image analysis. We investigate liver tissues characterized by antiinflammatory LMs which show a homogeneous distribution and increased cell numbers ...

We face a crisis in reproducibility, where it is impossible to believe most of the computational results shown in conferences and papers. Recent replication efforts and theoretical considerations indicate that most published research findings in biomedical research are wrong. This is especially problematic for the translation of computational models into the clinics. Modelling standards, software, and community initiatives are fundamental for reproducibility in systems biology and systems medicine. ...

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Introduction: The field of pharmacokinetics describe the kinetics of substances administered to the body, consisting of absorption, distribution, metabolization and elimination (ADME) of the substance. An important use case is the testing of liver function via test substances like caffeine, methacetin or galactose in dynamical liver function tests. Understanding the kinetics of test substances and drugs is crucial to evaluate therapeutic outcome and diagnostic value.

Results: Physiologically based ...

Pillar II dooley