Role of liver macrophages in different liver pathologies
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Liver macrophages (LMs) play a central role in acute and chronic liver pathologies. In our study, we sought to gain further insight into the role of LMs in different liver pathologies and into their characteristics after isolation from liver tissue. Here we will characterize LMs in human liver tissue sections using immunohistochemistry and bioinformatic image analysis. We investigate liver tissues characterized by antiinflammatory LMs which show a homogeneous distribution and increased cell numbers in correlation with chronic liver injuries. Detailed characterization of isolated macrophages revealed a complex disease dependent pattern of LMs consisting of pro- and anti-inflammatory macrophages of different origins, regulatory macrophages and monocytes.
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Created: 7th Apr 2021 at 16:23
Last updated: 12th May 2021 at 09:04
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The clinical department of Prof. Dr. med Daniel Seehofer performs more than 200 surgical interventions on the liver annually. Therefore, the group has access to a broad variety of liver tissues as well as corresponding patient, clinical laboratory and characterization data. I lead the experimental work group which has a longstandind expertise in the isolation, characterization and application of primary human liver and hepatoma cells. These cells as well as data generated from established liver ...
Projects: LiSyM Core Infrastructure and Management (LiSyM-PD), LiSyM Pillar I: Early Metabolic Injury (LiSyM-EMI), LiSyM Pillar II: Chronic Liver Disease Progression (LiSyM-DP), LiSyM Pillar III: Regeneration and Repair in Acute-on-Chronic Liver Failure (LiSyM-ACLF), LiSyM Pillar IV: Liver Function Diagnostics (LiSyM-LiFuDi), Model Guided Pharmacotherapy In Chronic Liver Disease (LiSyM-MGP), Molecular Steatosis - Imaging & Modeling (LiSyM-MSIM), The Hedgehog Signalling Pathway (LiSyM-JGMMS), Multi-Scale Models for Personalized Liver Function Tests (LiSyM-MM-PLF), LiSyM PALs, LiSyM network, LiSyM-Krebs Partnering
Institutions: HITS gGmbH
https://orcid.org/0000-0002-8683-7084
Data management and standardization expert for systems biology and systems medicine, responsible for the data management user requirements and user contacts within the German LiSyM network (Liver Systems Medicine: http://lisym.org/) and associated to the FAIRDOM team. Involved in different standardization initiatives and committees, i.e. COMBINE (http://co.mbine.org), ISO/TC 276 Biotechnology (https://www.iso.org/committee/4514241.html), European COST action CHARME (http://www.cost-charme.eu) and ...
Projects: The Hedgehog Signalling Pathway (LiSyM-JGMMS), LiSyM PALs, LiSyM network, LiSyM Scientific Leadership Team (LiSyM-LT), LiSyM-Krebs Partnering, SMART-NAFLD, DEEP-HCC network, Forschungsnetzwerk LiSyM-Krebs
Institutions: Universität Leipzig - Medizinische Fakultät - Institut für Biochemie, Universität Leipzig - Sächsischer Inkubator für Klinische Translation (SIKT)
Group leader of the junior group: The Hedgehog Signalling Pathway (LiSyM-JGMMS)
Liver Systems Medicine : striving to develop non-invasive methods for diagnosing and treating NAFLD by combining mathematical modeling and biological research. LiSyM, is a multidisciplinary research network, in which molecular and cell biologists, clinical researchers, pharmacologists and experts in mathematical modeling examine the liver in its entirety. LiSyM research focuses on the metabolic liver disease non-alcoholic fatty liver disease (NAFLD), which includes non-alcoholic steatohepatitis ...
Projects: LiSyM Core Infrastructure and Management (LiSyM-PD), LiSyM Pillar I: Early Metabolic Injury (LiSyM-EMI), LiSyM Pillar II: Chronic Liver Disease Progression (LiSyM-DP), LiSyM Pillar III: Regeneration and Repair in Acute-on-Chronic Liver Failure (LiSyM-ACLF), LiSyM Pillar IV: Liver Function Diagnostics (LiSyM-LiFuDi), Model Guided Pharmacotherapy In Chronic Liver Disease (LiSyM-MGP), Molecular Steatosis - Imaging & Modeling (LiSyM-MSIM), The Hedgehog Signalling Pathway (LiSyM-JGMMS), Multi-Scale Models for Personalized Liver Function Tests (LiSyM-MM-PLF), LiSyM PALs, Project Management PTJ, LiSyM network, LiSyM Scientific Leadership Team (LiSyM-LT)
Web page: https://www.lisym.org/
Dr. Madlen Matz-Soja (University of Leipzig) investigates the importance of a control mechanism - the hedgehog signaling pathway - for fatty liver disease. She has shown that the signaling pathway directs how liver cells in NAFLD accumulate fat. Hedgehog also affects sex hormones in the liver. Therefore, Matz-Soya hopes to clarify why women and men suffer from cirrhosis and liver cancer with varying degrees of frequency.
Programme: LiSyM: Liver Systems Medicine
Public web page: http://www.lisym.org/our-work/junior-group/hedgehog-influences
Start date: 1st Jan 2016
In this study, we investigated human LMs in tissue sections as well as isolated from human liver tissues which were characterized both in suspension and in adherent cultures to find out whether the inflammatory state of primary human LMs corresponds to patients and disease characteristics and whather it is possible to maintain the macrophage pattern in suspension as well as in adherent cultures with just a few limitations. (CCA: cholangiocarcinoma, CRLM: colorectal liver metastasis, FNH: focal ...
Submitter: René Hänsel
Investigation: Role of liver macrophages in different liver pa...
Assays: Characterization of liver macrophages (LMs) in human liver tissue sections
LMs were characterized in human liver tissue sections using immunohistochemistry and bioinformatic image analysis. Isolated cells were characterized in suspension using FACS analyses and in culture using
Submitter: René Hänsel
Assay type: Image Collection
Technology type: Imaging
Investigation: Role of liver macrophages in different liver pa...
Abstract (Expand)
Authors: Andrea Zimmermann, René Hänsel, Kilian Gemünden, Victoria Kegel-Hübner, Jonas Babel, Hendrik Bläker, Madlen Matz-Soja, Daniel Seehofer, Georg Damm
Date Published: 1st Apr 2021
Publication Type: Journal
