Cell atlas of the regenerating human liver after portal vein embolization

      The liver has the remarkable capacity to regenerate. In the clinic, this capacity can be induced by portal vein embolization (PVE), which redirects portal blood flow resulting in liver hypertrophy in locations with increased blood supply, and atrophy of embolized segments. Here we apply single-cell and single-nucleus transcriptomics on healthy, hypertrophied, and atrophied patient-derived liver samples to explore cell states in the liver during regeneration. We first establish an atlas of cell subtypes from the healthy human liver using fresh and frozen tissues, and then compare post-PVE samples with their reference counterparts. We find that PVE alters portal-central zonation of hepatocytes and endothelial cells. Embolization upregulates expression programs associated with development, cellular adhesion and inflammation across cell types. Analysis of interlineage crosstalk revealed key roles for immune cells in modulating regenerating tissue responses. Altogether, our data provides a rich resource for understanding homeostatic mechanisms arising during human liver regeneration and degeneration.

SEEK ID: https://seek.lisym.org/publications/408

DOI: 10.1101/2021.06.03.444016

Projects: LiSyM network

Publication type: Journal

Citation: biorxiv;2021.06.03.444016v1,[Preprint]

Date Published: 3rd Jun 2021

Registered Mode: by DOI

Authors: Agnieska Brazovskaja, Tomás Gomes, Christiane Körner, Zhisong He, Theresa Schaffer, Julian Connor Eckel, René Hänsel, Malgorzata Santel, Timm Denecke, Michael Dannemann, Mario Brosch, Jochen Hampe, Daniel Seehofer, Georg Damm, J. Gray Camp, Barbara Treutlein

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Brazovskaja, A., Gomes, T., Körner, C., He, Z., Schaffer, T., Eckel, J. C., Hänsel, R., Santel, M., Denecke, T., Dannemann, M., Brosch, M., Hampe, J., Seehofer, D., Damm, G., Camp, J. G., & Treutlein, B. (2021). Cell atlas of the regenerating human liver after portal vein embolization. In []. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2021.06.03.444016

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Created: 27th Nov 2023 at 12:20

Last updated: 8th Mar 2024 at 07:44

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