SOP: Mice hepatocytes isolation and co-cultivation with BMDM
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Created: 17th Apr 2024 at 08:35
Last updated: 6th May 2024 at 07:40
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Version 1 (earliest) Created 17th Apr 2024 at 08:35 by Stephanie Wolf
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Projects: LiSyM Pillar III: Regeneration and Repair in Acute-on-Chronic Liver Failure (LiSyM-ACLF), LiSyM Pillar II: Chronic Liver Disease Progression (LiSyM-DP), Forschungsnetzwerk LiSyM-Krebs, C-TIP-HCC network
Institutions: Universitätsklinikum Düsseldorf - Klinik für Gastroenterologie, Hepatologie und Infektiologie
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LiSyM-Krebs ist ein nationales Forschungsnetz zur Früherkennung und Prävention von Leberkrebs, das unter Verwendung des systemmedizinischen Forschungsansatzes die komplexen, dynamischen Prozesse der Krankheitsprogression analysiert, um ausgehend von den Erkenntnissen aus dem Forschungsnetz LiSyM die Entstehung von Leberkrebs besser zu verstehen, vorherzusagen und im besten Fall sogar zu verhindern. LiSyM-Krebs setzt die erfolgreichen Forschungsaktivitäten der BMBF-Vorgängerprogramme ...
Projects: LiSyM-Krebs Partnering, Forschungsnetzwerk LiSyM-Krebs, SMART-NAFLD, DEEP-HCC network, C-TIP-HCC network
Web page: https://www.lisym-cancer.org
C-TIP-HCC- Mechanism-based Multiscale Model to Dissect the Tipping Point from Liver Cirrhosis to Hepatocellular Carcinoma
The ultimate goal of the C-TIP-HCC is a „mechanistic multiscale model to describe dynamic changes in regenerative nodes across a tipping point (TIP) towards development in patients with cirrhosis to facilitate early monitoring and intervention“ and facilitate intervention“. To achieve this, we will conduct in-depth studies on cirrhotic regenerative nodes.
Programme: LiSyM-Krebs - Systemmedizinisches Forschungsnetz zur Früherkennung und Prävention von Leberkrebs
Public web page: Not specified
Organisms: Homo sapiens, Mus musculus
Macrophages play an important role in maintaining liver homeostasis and regeneration. Here we investigate how different macrophage populations of the liver differ in terms of their activation state and which other liver cell populations may play a role in regulating the same.
Submitter: Stephanie Wolf
Studies: Study changes in gene expressionand in the activation state of macrophag...
Assays: Hepatocytes induces properties of wound healing type and regulatory macr..., Transcriptomics : RNA Microarray, Transcriptomics: RNA-seq of coding RNA from single cells
Snapshots: No snapshots
Reverse transcription PCR, flow cytometry, transcriptome, proteome, secretome, single cell analysis, and immunohistochemical methods were used to study changes in gene expression as well as the activation state of macrophages in vitro and in vivo under homeostatic conditions and after partial hepatectomy.
Submitter: Stephanie Wolf
Investigation: Hepatocytes reprogram liver macrophages involvi...
Assays: Hepatocytes induces properties of wound healing type and regulatory macr..., Transcriptomics : RNA Microarray, Transcriptomics: RNA-seq of coding RNA from single cells
Snapshots: No snapshots
The liver comprises the body´s largest pool of macrophages constituting approximately 80 % of all sessile tissue macrophages of the body. After liver damage monocyte-derived macrophages are recruited into the liver and were here affected by a micro milieu which is considerably influenced by hepatocytes. Up to now the complex network that determines the differentiation and function of liver macrophages and the impact of the intercellular communication between macrophages and the other parenchymal ...
Submitter: Stephanie Wolf
Assay type: Shotgun proteomics
Technology type: LTQ Orbitrap Elite
Investigation: Hepatocytes reprogram liver macrophages involvi...
Organisms: Mus musculus
SOPs: SOP: Mice hepatocytes isolation and co-cultivat... and 1 hidden item
Data files: Shotgun proteomics data at PRIDE
Snapshots: No snapshots
We describe a distinct macrophage population, whose presence in the liver during homoeostasis depends on recruitment signals mediated by the chemokine receptor CCR2. The identified polarization state of this population closely resembles that induced in co-culture experiments, where hepatocytes are reducing the availability of TGFb to macrophages. Accordingly, disruption of TGFb signal transduction in macrophages phenocopies the influence of hepatocytes on macrophage polarization.
Submitter: Stephanie Wolf
Assay type: Transcriptomics
Technology type: Technology Type
Investigation: Hepatocytes reprogram liver macrophages involvi...
Organisms: No organisms
SOPs: SOP: Mice hepatocytes isolation and co-cultivat...
Data files: Sequnce data at ENA, The single-cell RNA sequencing data (ArrayExpress)
Snapshots: No snapshots
Transcriptomics: the RNA expression of primary hepatocytes and BMDM (Bone Marrow–Derived acrophages) were analysed in 4 different conditions (mono- vs. co-culture, hepatocytes and macrophages) with 5 replicas each to detect the impact of hepatocytes on macrophages and vice versa.
Submitter: Stephanie Wolf
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Hepatocytes reprogram liver macrophages involvi...
Organisms: No organisms
SOPs: SOP: Mice hepatocytes isolation and co-cultivat...
Data files: GEO data set, The single-cell RNA sequencing data (ArrayExpress)
Snapshots: No snapshots