Hepatocytes induces properties of wound healing type and regulatory macrophages in co-cultivated BMDM
The liver comprises the body´s largest pool of macrophages constituting approximately 80 % of all sessile tissue macrophages of the body. After liver damage monocyte-derived macrophages are recruited into the liver and were here affected by a micro milieu which is considerably influenced by hepatocytes. Up to now the complex network that determines the differentiation and function of liver macrophages and the impact of the intercellular communication between macrophages and the other parenchymal and non-parenchymal cell types of the liver is not well understood. The present study investigates the role of the intercellular communication between macrophages and hepatocytes on macrophage differentiation and function. Under physiological conditions the sinusoidal endothelial cell layer and the space of Dissé separate macrophages and hepatocytes from each other. Therefore the study focuses on the impact of the intercellular communication via soluble mediators on the differentiation of bone marrow derived macrophages (BMDM), which were recruited to the liver after liver injury, in co-culture models with highly purified primary murine hepatocytes embedded in a sandwich collagen matrix.
SEEK ID: https://seek.lisym.org/assays/275
Experimental assay
Projects: C-TIP-HCC network, Forschungsnetzwerk LiSyM-Krebs
Investigation: Hepatocytes reprogram liver macrophages involving control of TGF-β activation, influencing liver regeneration and injury.
Assay position:
Assay type: Shotgun proteomics
Technology type: LTQ Orbitrap Elite
Organisms: Mus musculus
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Submitter
Views: 118
Created: 2nd Aug 2023 at 08:54
Last updated: 6th May 2024 at 07:40
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