Basal MET phosphorylation is an indicator of hepatocyte dysregulation in liver disease

Abstract:
        Abstract
        Chronic liver diseases are worldwide on the rise. Due to the rapidly increasing incidence, in particular in Western countries, metabolic dysfunction-associated steatotic liver disease (MASLD) is gaining importance as the disease can develop into hepatocellular carcinoma. Lipid accumulation in hepatocytes has been identified as the characteristic structural change in MASLD development, but molecular mechanisms responsible for disease progression remained unresolved. Here, we uncover in primary hepatocytes from a preclinical model fed with a Western diet (WD) an increased basal MET phosphorylation and a strong downregulation of the PI3K-AKT pathway. Dynamic pathway modeling of hepatocyte growth factor (HGF) signal transduction combined with global proteomics identifies that an elevated basal MET phosphorylation rate is the main driver of altered signaling leading to increased proliferation of WD-hepatocytes. Model-adaptation to patient-derived hepatocytes reveal patient-specific variability in basal MET phosphorylation, which correlates with patient outcome after liver surgery. Thus, dysregulated basal MET phosphorylation could be an indicator for the health status of the liver and thereby inform on the risk of a patient to suffer from liver failure after surgery.

SEEK ID: https://seek.lisym.org/publications/412

DOI: 10.1038/s44320-023-00007-4

Projects: Forschungsnetzwerk LiSyM-Krebs, SMART-NAFLD

Publication type: Journal

Journal: Molecular Systems Biology

Citation: Mol Syst Biol

Date Published: 12th Jan 2024

Registered Mode: by DOI

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Citation
Burbano de Lara, S., Kemmer, S., Biermayer, I., Feiler, S., Vlasov, A., D’Alessandro, L. A., Helm, B., Mölders, C., Dieter, Y., Ghallab, A., Hengstler, J. G., Körner, C., Matz-Soja, M., Götz, C., Damm, G., Hoffmann, K., Seehofer, D., Berg, T., Schilling, M., … Klingmüller, U. (2024). Basal MET phosphorylation is an indicator of hepatocyte dysregulation in liver disease. In Molecular Systems Biology (Vol. 20, Issue 3, pp. 187–216). Springer Science and Business Media LLC. https://doi.org/10.1038/s44320-023-00007-4
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Created: 19th Jan 2024 at 12:34

Last updated: 8th Mar 2024 at 07:44

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