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Supplmentary Materials, Methods, Figures and Tables

Creator: Seddik Hammad

Submitter: Seddik Hammad

List of used antibodies

Creator: Seddik Hammad

Submitter: Seddik Hammad

Publications in which periportal fibrosis has been reported after repeated doses of CCl4.

Creator: Seddik Hammad

Submitter: Seddik Hammad

Materials and Methods

Creator: Seddik Hammad

Submitter: Seddik Hammad

CYP2E1 activity in HepG2-E47 was measured. TAM inhibits (not significantly) the CYP2E1 activity compared to alcohol and DMSO-treated cells. C34 cell line was used as a negative control. Bars represent the means±SD of 3 biological replica. Student’s t test (paired t test) was used to check the statistical differences upon TAM incubation (JPG 1530 KB)

Creator: Seddik Hammad

Submitter: Seddik Hammad

Protein level of CYP2E1 was analyzed by co-immunofluorescence staining immediately after attachment (3h) and 24 hours later. b and c) Protein and mRNA levels of CYP2E1 were analyzed upon TAM pretreatment. d and e) Protein and mRNA levels of catalase were assessed upon TAM pretreatment. Bars represent the means±SD of 3 mice (JPG 3000 KB)

Creator: Seddik Hammad

Submitter: Seddik Hammad

Formalin-fixed livers were processed and stained with HE, CYP2E1, GS, F4/80 and CD45 to visualize and quantify hepatocellular necrosis, metabolizing enzymes, resident macrophages and recruited immune cells, respectively. Slides were scanned and the signal was randomly quantified in 15 images per liver. Yellow dashed lines indicate the necrotic areas. Yellow arrows refer to the positive signals. Scale bars are 200µm for HE and CYP2E1 and 100µm for GS, F4/80 and CD45 (JPG 6105 KB)

Creator: Seddik Hammad

Submitter: Seddik Hammad

No description specified

Creator: Seddik Hammad

Submitter: Seddik Hammad

RNA microarray made with primary hepatocytes from female mice with hepatocyte-specific, embryonic knockout of Smoothened

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