2 items tagged with 'Lipidomics'.
Former postdoc in the Theoretical Biophysics group headed by Edda Klipp at Humboldt-Universität zu Berlin and member of the LiSyM network, I am now an independent Group Leader at the Technical University of Munich focusing on developing an advanced bioinformatics toolbox for analyzing, integrating, and mining of various omics data in the context of precision medicine as well as molecular disease and patient subtyping.
Abstract (Expand)
Shotgun analysis provides a quantitative snapshot of the lipidome composition of cells, tissues, or model organisms; however, it does not elucidate the spatial distribution of lipids. Here we demonstrate … that shotgun analysis could quantify low-picomole amounts of lipids isolated by laser capture microdissection (LCM) of hundred micrometer-sized histological zones visualized at the cryosections of tissues. We identified metabolically distinct periportal (pp) and pericentral (pc) zones by immunostaining of 20 mum thick cryosections of a healthy mouse liver. LCM was used to ablate, catapult, and collect the tissue material from 10 to 20 individual zones covering a total area of 0.3-0.5 mm(2) and containing ca. 500 cells. Top-down shotgun profiling relying upon computational stitching of 61 targeted selective ion monitoring ( t-SIM) spectra quantified more than 200 lipid species from 17 lipid classes including glycero- and glycerophospholipids, sphingolipids, cholesterol esters, and cholesterol. Shotgun LCM revealed the overall commonality of the full lipidome composition of pp and pc zones along with significant ( p < 0.001) difference in the relative abundance of 13 lipid species. Follow-up proteomics analyses of pellets recovered from an aqueous phase saved after the lipid extraction identified 13 known and 7 new protein markers exclusively present in pp or in pc zones and independently validated the specificity of their visualization, isolation, and histological assignment.
Authors: O. Knittelfelder, S. Traikov, O. Vvedenskaya, A. Schuhmann, S. Segeletz, A. Shevchenko, A. Shevchenko
Date Published: 30th Jul 2018
Publication Type: Not specified
PubMed ID: 30004672
Citation: Anal Chem. 2018 Jul 30. doi: 10.1021/acs.analchem.8b02004.
Created: 1st Aug 2018 at 07:08