Confounding influence of tamoxifen in mouse models of Cre recombinase-induced gene activity or modulation


Tamoxifen (TAM) is commonly used for cell type specific Cre recombinase-induced gene inactivation and in cell fate tracing studies. Inducing a gene knockout by TAM and using non-TAM exposed mice as controls lead to a situation where differences are interpreted as consequences of the gene knockout but in reality result from TAM-induced changes in hepatic metabolism. The degree to which TAM may compromise the interpretation of animal experiments with inducible gene expression still has to be elucidated. Here, we report that TAM strongly attenuates CCl4-induced hepatotoxicity in male C57Bl/6N mice, even after a 10 days TAM exposure-free period. TAM decreased (p 


DOI: 10.1007/s00204-018-2254-4

Projects: LiSyM network

Publication type: Not specified

Journal: Arch Toxicol

Citation: Arch Toxicol 19(11) : 715

Date Published: 4th Jul 2018

Registered Mode: Not specified

Authors: Seddik Hammad, Amnah Othman, Christoph Meyer, Ahmad Telfah, Joerg Lambert, Bedair Dewidar, Julia Werle, Zeribe Chike Nwosu, Abdo Mahli, Christof Dormann, Yan Gao, Kerry Gould, Mei Han, Xiaodong Yuan, Mikheil Gogiashvili, Roland Hergenröder, Claus Hellerbrand, Maria Thomas, Matthias Philip Ebert, Salah Amasheh, Jan G. Hengstler, Steven Dooley

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Hammad, S., Othman, A., Meyer, C., Telfah, A., Lambert, J., Dewidar, B., Werle, J., Nwosu, Z. C., Mahli, A., Dormann, C., Gao, Y., Gould, K., Han, M., Yuan, X., Gogiashvili, M., Hergenröder, R., Hellerbrand, C., Thomas, M., Ebert, M. P., … Dooley, S. (2018). Confounding influence of tamoxifen in mouse models of Cre recombinase-induced gene activity or modulation. In Archives of Toxicology (Vol. 92, Issue 8, pp. 2549–2561). Springer Science and Business Media LLC.

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Created: 7th Jul 2018 at 19:19

Last updated: 7th Jul 2018 at 19:19

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