Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry.

Abstract:

Clinical lipidomics is an emerging biomarker discovery approach that compares lipid profiles under pathologically and physiologically normal conditions. Here we describe a method for the absolute (molar) quantification of more than 200 molecules from 14 major lipid classes from 5 muL of human blood plasma using high-resolution top-down shotgun mass spectrometry. Because of its technical simplicity and robustness, the protocol lends itself for high-throughput clinical lipidomics screens.

SEEK ID: https://seek.lisym.org/publications/51

PubMed ID: 28674888

Projects: LiSyM Pillar I: Early Metabolic Injury (LiSyM-EMI)

Publication type: Not specified

Journal: Methods Mol Biol

Citation: Methods Mol Biol. 2017;1619:203-212. doi: 10.1007/978-1-4939-7057-5_16.

Date Published: 4th Jul 2017

Registered Mode: Not specified

Authors: S. Sales, O. Knittelfelder, A. Shevchenko

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Views: 3739

Created: 8th Jan 2018 at 10:42

Last updated: 8th Mar 2024 at 07:44

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