Clinical lipidomics is an emerging biomarker discovery approach that compares lipid profiles under pathologically and physiologically normal conditions. Here we describe a method for the absolute (molar) quantification of more than 200 molecules from 14 major lipid classes from 5 muL of human blood plasma using high-resolution top-down shotgun mass spectrometry. Because of its technical simplicity and robustness, the protocol lends itself for high-throughput clinical lipidomics screens.
SEEK ID: https://seek.lisym.org/publications/51
PubMed ID: 28674888
Projects: LiSyM Pillar I: Early Metabolic Injury (LiSyM-EMI)
Publication type: Not specified
Journal: Methods Mol Biol
Citation: Methods Mol Biol. 2017;1619:203-212. doi: 10.1007/978-1-4939-7057-5_16.
Date Published: 4th Jul 2017
Registered Mode: Not specified
Views: 4136
Created: 8th Jan 2018 at 10:42
Last updated: 8th Mar 2024 at 07:44
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