Intravital multi-modal flux analysis reveals the transport mechanism of bile acids through hepatic microconduits

Abstract:

Small-molecule flux in tissue-microdomains is essential for organ function, but knowledge of this process is scant due to the lack of suitable methods applicable to live animals. We developed a methodology based on dynamic and correlative imaging for quantitative intravital flux analysis. Application to the liver, challenged the prevailing ‘mechano-osmotic’ theory of canalicular bile flow. After active transport across hepatocyte membranes bile salts are transported in the canaliculi primarily by diffusion. Only in the interlobular ducts, diffusion is augmented by regulatable advection. We corroborate these observations with in silico simulations and pan-species comparisons of lobule size. This study demonstrates a flux mechanism, where the energy invested in transmembrane transport entropically dissipates in a sub-micron scale vessel network.

Citation: Intravital multi-modal flux analysis reveals the transport mechanism of bile acids through hepatic microconduits 63 : 951

Date Published: No date defined

Authors: Nachiket Vartak, Georgia Guenther, Florian Joly, Amruta Damle-Vartak, Gudrun Wibbelt, Jörns Fickel, Simone Jörs, Brigitte Begher-Tibbe, Adrian Friebel, Kasimir Wansing, Ahmed Ghallab, Marie Rosselin, Noemie Boissier, Irene Vignon-Clementel, Christian Hedberg, Fabian Geisler, Heribert Hofer, Peter Jansen, Stefan Hoehme, Dirk Drasdo, Jan Hengstler

Help
help Creator
Citation
Vartak, N., Guenther, G., Joly, F., Damle-Vartak, A., Wibbelt, G., Fickel, J., … Hengstler, J. G. (2019, September 26). Intravital multi-modal flux analysis reveals the transport mechanism of bile acids through hepatic microconduits. []. Cold Spring Harbor Laboratory. http://doi.org/10.1101/778803
Activity

Views: 85

Created: 4th Feb 2020 at 07:24

Last updated: 4th Feb 2020 at 07:26

help Attributions

None

Related items

Powered by
(v.1.9.0)
Copyright © 2008 - 2019 The University of Manchester and HITS gGmbH