Human RNA-Seq data set GSM2819728 stored in NCBI (GEO)
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Created: 28th Jan 2022 at 16:31
Last updated: 2nd Feb 2022 at 07:48
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Version 1 (earliest) Created 28th Jan 2022 at 16:31 by Mario Brosch
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Liver Systems Medicine : striving to develop non-invasive methods for diagnosing and treating NAFLD by combining mathematical modeling and biological research. LiSyM, is a multidisciplinary research network, in which molecular and cell biologists, clinical researchers, pharmacologists and experts in mathematical modeling examine the liver in its entirety. LiSyM research focuses on the metabolic liver disease non-alcoholic fatty liver disease (NAFLD), which includes non-alcoholic steatohepatitis ...
Projects: LiSyM Core Infrastructure and Management (LiSyM-PD), LiSyM Pillar I: Early Metabolic Injury (LiSyM-EMI), LiSyM Pillar II: Chronic Liver Disease Progression (LiSyM-DP), LiSyM Pillar III: Regeneration and Repair in Acute-on-Chronic Liver Failure (LiSyM-ACLF), LiSyM Pillar IV: Liver Function Diagnostics (LiSyM-LiFuDi), Model Guided Pharmacotherapy In Chronic Liver Disease (LiSyM-MGP), Molecular Steatosis - Imaging & Modeling (LiSyM-MSIM), The Hedgehog Signalling Pathway (LiSyM-JGMMS), Multi-Scale Models for Personalized Liver Function Tests (LiSyM-MM-PLF), LiSyM PALs, Project Management PTJ, LiSyM network, LiSyM Scientific Leadership Team (LiSyM-LT)
Web page: https://www.lisym.org/
One of the tasks of the healthy liver is to store fat. Yet, at some stage, too much fat makes the liver sick. One critical time point occurs when a healthy fatty liver becomes inflamed and progresses to steatohepatitis, or NASH. LiSyM-Pillar I will identify what events lead to this transition. Does it occur in all parts of the liver? Which molecules indicate that it is taking place? Can the degeneration be stopped or undone - and if so, how?
Programme: LiSyM: Liver Systems Medicine
Public web page: http://www.lisym.org/our-work/pillar-research/zones-of-the-liver
Start date: 1st Jan 2016
Organisms: Mus musculus, Rattus rattus, Rattus norvegicus, Homo sapiens
To investigate the underlying molecular principles of metabolic and morphogenic zonation of the human liver lobule, we generated an integrated epigenetic map across three zones (pericentral, intermediate and periportal) by methylation and transcriptomic analysis of hepatocytes captured by laser micro-dissection. We observe a deep link between epigenetic zonation of human liver and a zonated expression of metabolic and morphogenic pathways: Key transcriptionally zonated enzymes in xenobiotic and ...
Submitter: Mario Brosch
Studies: Integrated epigenetic map across three hepatic zones (pericentral, inte...
Assays: DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., Transcriptomic analysis of hepatocytes ( hepatic zone: intermediate (IZ), Transcriptomic analysis of hepatocytes ( hepatic zone: periportal (PP), Transcriptomic analysis of hepatocytes ; hepatic zone: pericentral (CV)
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The study comprises 19 human liver biopsy donors divided into the groups normal control (NC = 7012, 7173, 7194, 7279), healthy obese (HO = 6758, 6922, 7213, 7230, 7252), bland steatosis (STEA = 6967, 7137, 7172, 7181, 7251) and early NASH (EARLY = 6610, 7041, 7157, 7188, 7344).
Hepatocytes captured by laser microdissection were obtained from three hepatic zones (pericentral, intermediate and periportal) and subjected to reduced representation bisulfite sequencing and RNA-seq resulting in 114 ...
Submitter: Mario Brosch
Investigation: Epigenomic analysis of micro-dissected human liver
Assays: DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., DNA-methylation at binding sites of uniformly expressed transcription fa..., Transcriptomic analysis of hepatocytes ( hepatic zone: intermediate (IZ), Transcriptomic analysis of hepatocytes ( hepatic zone: periportal (PP), Transcriptomic analysis of hepatocytes ; hepatic zone: pericentral (CV)
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Transcriptomic analysis of hepatocytes captured by laser micro-dissection from hepatic zone: periportal (PP)
Submitter: Mario Brosch
Assay type: RNA-seq Profiling
Technology type: Rna-seq
Investigation: Epigenomic analysis of micro-dissected human liver
Organisms: Homo sapiens
SOPs: SOP : liver tissue microdissection for RNA seq...
Data files: Human RNA-Seq data set GSM2819715 stored in..., Human RNA-Seq data set GSM2819716 stored in..., Human RNA-Seq data set GSM2819717 stored in..., Human RNA-Seq data set GSM2819718 stored in..., Human RNA-Seq data set GSM2819719 stored in..., Human RNA-Seq data set GSM2819720 stored in..., Human RNA-Seq data set GSM2819721 stored in..., Human RNA-Seq data set GSM2819722 stored in..., Human RNA-Seq data set GSM2819723 stored in..., Human RNA-Seq data set GSM2819724 stored in..., Human RNA-Seq data set GSM2819725 stored in..., Human RNA-Seq data set GSM2819726 stored in..., Human RNA-Seq data set GSM2819727 stored in..., Human RNA-Seq data set GSM2819728 stored in..., Human RNA-Seq data set GSM2819729 stored in..., Human RNA-Seq data set GSM2819730 stored in..., Human RNA-Seq data set GSM2819731 stored in..., Human RNA-Seq data set GSM2819732 stored in..., Human RNA-Seq data set GSM2819733 stored in...
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