Assays
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The liver comprises the body´s largest pool of macrophages constituting approximately 80 % of all sessile tissue macrophages of the body. After liver damage monocyte-derived macrophages are recruited into the liver and were here affected by a micro milieu which is considerably influenced by hepatocytes. Up to now the complex network that determines the differentiation and function of liver macrophages and the impact of the intercellular communication between macrophages and the other parenchymal ...
Submitter: Stephanie Wolf
Assay type: Shotgun proteomics
Technology type: LTQ Orbitrap Elite
Investigation: Hepatocytes reprogram liver macrophages involvi...
We describe a distinct macrophage population, whose presence in the liver during homoeostasis depends on recruitment signals mediated by the chemokine receptor CCR2. The identified polarization state of this population closely resembles that induced in co-culture experiments, where hepatocytes are reducing the availability of TGFb to macrophages. Accordingly, disruption of TGFb signal transduction in macrophages phenocopies the influence of hepatocytes on macrophage polarization.
Submitter: Stephanie Wolf
Assay type: Transcriptomics
Technology type: Technology Type
Investigation: Hepatocytes reprogram liver macrophages involvi...
Transcriptomics: the RNA expression of primary hepatocytes and BMDM (Bone Marrow–Derived acrophages) were analysed in 4 different conditions (mono- vs. co-culture, hepatocytes and macrophages) with 5 replicas each to detect the impact of hepatocytes on macrophages and vice versa.
Submitter: Stephanie Wolf
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Hepatocytes reprogram liver macrophages involvi...
RNA sequence data
Submitter: Olga Krebs
Assay type: RNA-seq Profiling
Technology type: Technology Type
Investigation: LiSyM-Cancer project data for integration into...
Study: GENOMICS/TRANSCRIPTOMICS
DNA sequence data
Submitter: Olga Krebs
Assay type: Transcriptomics
Technology type: Technology Type
Investigation: LiSyM-Cancer project data for integration into...
Study: GENOMICS/TRANSCRIPTOMICS
Western blot
Submitter: Olga Krebs
Assay type: Immune Precipitation
Technology type: Technology Type
Investigation: LiSyM-Cancer project data for integration into...
Study: PROTEOMICS
Submitter: Olga Krebs
Biological problem addressed: Signal Induction
Investigation: Investigation of the Smad Protein Complex Forma...
exp assay I Lucarelli paper
Submitter: Olga Krebs
Assay type: Proteomics
Technology type: Mass Spectrometry
Investigation: Investigation of the Smad Protein Complex Forma...
DMPlaning 2022
Submitter: Olga Krebs
Biological problem addressed: Model Analysis Type
Investigation: 1 hidden item
Study: 1 hidden item
Transcriptomic analysis of hepatocytes captured by laser micro-dissection from hepatic zone: periportal (PP)
Submitter: Mario Brosch
Assay type: RNA-seq Profiling
Technology type: Rna-seq
Investigation: Epigenomic analysis of micro-dissected human liver
Transcriptomic analysis of hepatocytes captured by laser micro-dissection from hepatic zone: intermediate (IZ)
Submitter: Mario Brosch
Assay type: RNA-seq Profiling
Technology type: Rna-seq
Investigation: Epigenomic analysis of micro-dissected human liver
DNA-methylation at binding sites of uniformly expressed transcription factors in human hepatocytes ; hepatic zone: intermediate (IZ)
Submitter: Olga Krebs
Assay type: Methylation Profiling
Technology type: Technology Type
Investigation: Epigenomic analysis of micro-dissected human liver
Transcriptomic analysis of hepatocytes captured by laser micro-dissection from hepatic zone: pericentral (CV)
Submitter: Mario Brosch
Assay type: RNA-seq Profiling
Technology type: Rna-seq
Investigation: Epigenomic analysis of micro-dissected human liver
DNA-methylation at binding sites of uniformly expressed transcription factors in human hepatocytes ; hepatic zone: pericentral (CV)
Submitter: Mario Brosch
Assay type: Methylation Profiling
Technology type: Technology Type
Investigation: Epigenomic analysis of micro-dissected human liver
DMPlaning 2021
Submitter: Olga Krebs
Biological problem addressed: Model Analysis Type
Investigation: 1 hidden item
Study: 1 hidden item